Dear Rita,

There are now more sensitive and selective modifications
for staining mercury with Timm's sulphide-silver method.

Method:
Danscher G & Rungby J 1986. Histochem J 18:109-114.
Some aplications:
Arvidson B 1992. Muscle & Nerve 15:1089-1094.
Pamphlett R & Waley P 1996. J Neurol Sci 135:63-67.
Pamphlett R & Kum-Jew S 2001. Neurotoxicol Teratol
23:191-196.

There may be more recent variations of the method,
probably with Danscher as chief author. Try Google
or Pubmed - or a cited ref search with Web of Science
if that is available to you. It should be, at a
big place like U of C.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________

"GUTIERREZ, JUAN" wrote:
> 
> I found a reference for Voight's modification of the Timm silver sulfide method that is specific for mercury. (Acta Histochem.  14:315, 1962).
> I found the reference in Lillie's Histopathologic Technic and Practical Histochemistry, Third Edition 1965.  An oldie but a goodie.  Good luck.
> 
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
> 
> My opinions are my own and do not reflect those of my employer.  Long live free speech!
> 
> -----Original Message-----
> From: Rita Angel [mailto:RITA.ANGEL@UC.EDU]
> Sent: Wednesday, December 08, 2004 11:19 AM
> To: histonet@pathology.swmed.edu
> Subject: [Histonet] stain that binds mercury
> 
> Hi everyone,
> 
> We have an investigator here that is interested in finding a stain that
> will localize mercury in sections of cicadas. Does anyone know of anything
> that will bind mercury in tissues? This is all the information I have at
> this point.
> 
> Thank you for your help and expertise,
> 
> Rita Angel, HT
> University of Cincinnati
> 
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