Re: [Histonet] AFB staining

From:Gayle Callis

You distilled water in waterbath may be the source.  There are saprophitic 
Mycobacteria that grow in distilled water when you store your water in 
plastic carboys, and you pick up these bacteri when you pick up the 
sections.   This has been published and also discussed in Histonet - check 
the archives. Be sure you wash plastic distilled water carboys frequently 
and use Clorox, followed by some good rinses.   If distilled water is made 
in another area with huge storage tanks, the tanks should also be on a 
regular cleaning schedule including all tubing.

  I don't recall that the organisms come from tap water, but that also may 
be the case.

   At 07:13 PM 12/10/2004, you wrote:
>Hi Everyone,
>We are having trouble with false positive AFB staining. Positive rods are 
>staining on charged slides outside the tissue. The solution is filtered 
>and run down on a Sakura H&E stainer.
>Any suggestions are very welcome.
>Eileen C. Dusek
>Edward Hospital
>Do you Yahoo!?
>  Yahoo! Mail - 250MB free storage. Do more. Manage less.
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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