Re: [Histonet] AFB staining
You distilled water in waterbath may be the source. There are saprophitic
Mycobacteria that grow in distilled water when you store your water in
plastic carboys, and you pick up these bacteri when you pick up the
sections. This has been published and also discussed in Histonet - check
the archives. Be sure you wash plastic distilled water carboys frequently
and use Clorox, followed by some good rinses. If distilled water is made
in another area with huge storage tanks, the tanks should also be on a
regular cleaning schedule including all tubing.
I don't recall that the organisms come from tap water, but that also may
be the case.
At 07:13 PM 12/10/2004, you wrote:
>We are having trouble with false positive AFB staining. Positive rods are
>staining on charged slides outside the tissue. The solution is filtered
>and run down on a Sakura H&E stainer.
>Any suggestions are very welcome.
>Eileen C. Dusek
>Do you Yahoo!?
> Yahoo! Mail - 250MB free storage. Do more. Manage less.
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