Nice and red?



-----Original Message-----
From: Kathleen Roberts [mailto:kgrobert@rci.rutgers.edu] 
Sent: 01 December 2004 14:35
To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Ammonia water pH and other bluing
solutions[Scanned]

Heck, we still use that.  All our slides look lovely.

The mellow one-
Kathleen Roberts
Rutgers University

Jackie M O'Connor wrote:

>Anyone remember using saturated lithium carbonate?  I could probably use a 
>little lithium right now . . . . . 
>
>Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step just 
>bringing the slides BACK to a neutral pH after treating them with acid 
>which makes them purple-ish?  I like the ammonia because it 'seems' to 
>make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to 
>make them "blah".(Perception is in the eye of the beholder)   Running tap 
>water will make the same miracle happen, but it takes longer.
>
>
>
>
>
>
>"Johnson, Teri" 
>Sent by: histonet-bounces@lists.utsouthwestern.edu
>11/30/2004 12:51 PM
>
> 
>        To:     "Histonet" 
>        cc: 
>        Subject:        [Histonet] Re: Ammonia water pH and other bluing
solutions
>
>
>Way back in the day, Brigati was using PBS on his immunostainer to blue
>after the hematoxylin counterstain.  I have since used PBS to blue my
>slides instead of ammonium hydroxide/water.  It's cheap, and very easy
>on the sections, and I have plenty of it on hand.
> 
>
>Teri Johnson
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri  64110
>tjj@stowers-institute.org
>
>
> 
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>

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