RE: [Histonet] Re: Ammonia water pH and other bluing solutions[Sc anned]

From:Kemlo Rogerson

I think you are wrong, so I'll correct you, as you requested.

I thought the 'blue' state of haematin was, like litmus, the effect an
alkaline condition has on the siderphilic dye. Red, like litmus, denoted
acidic conditions. Damned if I know what colour (with the 'u') haematin goes
when neutral (bluey/ red?)

Running tap water takes longer, I concede, but good things are worth waiting
for and it's easier to make up; we rush too much I find. TIP: Never use
London water, been through too many kidneys!

-----Original Message-----
From: Jackie M O'Connor [mailto:Jackie.O'] 
Sent: 30 November 2004 19:09
To: Johnson, Teri
Cc: Histonet;
Subject: Re: [Histonet] Re: Ammonia water pH and other bluing

Anyone remember using saturated lithium carbonate?  I could probably use a 
little lithium right now . . . . . 

Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step just 
bringing the slides BACK to a neutral pH after treating them with acid 
which makes them purple-ish?  I like the ammonia because it 'seems' to 
make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to 
make them "blah".(Perception is in the eye of the beholder)   Running tap 
water will make the same miracle happen, but it takes longer.

"Johnson, Teri" 
Sent by:
11/30/2004 12:51 PM

        To:     "Histonet" 
        Subject:        [Histonet] Re: Ammonia water pH and other bluing

Way back in the day, Brigati was using PBS on his immunostainer to blue
after the hematoxylin counterstain.  I have since used PBS to blue my
slides instead of ammonium hydroxide/water.  It's cheap, and very easy
on the sections, and I have plenty of it on hand.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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