We use Gomori's trichrome on frozen sections.  It's a one step procedure that gives you a faster answer.  Are you able to try alternatives to MTS?
Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live free speech!


-----Original Message-----
From: Corinne Willmott [mailto:corinnewillmott@fusemail.com] 
Sent: Thursday, December 02, 2004 9:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Masson's Trichrome on frozen heart sections

Can anyone tell me if it is possible to use Masson's Trichrome stain on
frozen cardaic sections and if so is there a recommended protocol? I am
new to histology and all the references I've found so far refer to
paraffin embedded sections.
Thanks

Corinne Willmott
Stem Cell Research
National Blood Service
John Radcliffe Hospital
Headington
Oxford


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