RE: [Histonet] IHC ~ Tissue Falling Off Slides
|From:||"Christine Tambasco" |
I use poly-l-lysine slides for immunos (i make them myself) . Also i
use fisher's probe slides and the citra retreival i do in the
microwave. I put the slides in a plastic slide mailer with citra -
microwave on high for 45sec - 1min then I add more citra and microwave
3 minutes at 50% power. It works for me. Good Luck!
Christine Tambasco, HT (ASCP)
St. Mary's Hospital , Amsterdam, New York ph-5188417287
>From: "Jones, Laura"
>To: "Histonet (E-mail)"
>Subject: [Histonet] IHC ~ Tissue Falling Off Slides
>Date: Thu, 9 Dec 2004 11:03:38 -0500
>Hello fellow Histonetters.
>We have suddenly started to have problems with tissue falling off
>during IHC processing. We suspect the problem may be our slides,
>we have been using the same ones from Mercedes Medical for a while
>have worked beautifully! We have contacted them, and they are
>and very kindly replacing our stock, but in the meantime, our
>has asked me to ask all of you where else we can find 25 X 75
>slides. We use the Zymed ST-5050 automated stainer, and the 1 X 3
>are sometimes a bit to big to fit on the carousel.
>Just for additional info, we seem to be experiencing problems mostly
>the more alkaline retreival solutions - AR-10 (Biogenex) and EDTA
>and Trilogy (Cell Marque). We use the Black & Decker steamer, and
>adjusted our times in retrieval from 75 minutes (!) to 20 minutes,
>Pathologist direction. We have also tried 20 minutes heating, then
>the slides for 20 minutes of boiling, then allowing them to stand for
>Thanks in advance for all of your knowledge!
>The Histochicks at Sharon Regional
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