RE: [Histonet] ER staining problem

From:"Stapf, Ross"

Are your slides cut fresh, or are they pre-cut days or weeks ahead?  ER
is one antibody some people have problems with if it is not cut fresh. 

Has something changed in the fixation? 

These are the only ideas I have on the subject right now.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246

214-820-4110 fax

-----Original Message-----
[] On Behalf Of Chris
Sent: Wednesday, December 08, 2004 10:39 AM
Subject: [Histonet] ER staining problem

I need your help.
I use a Ventana ES and am having a real problem getting the ER antibody
to work.

It was always a great result, however in the last 6 months or so it has
gotten worse to the point now where the stain is dead negative. I've
tried different slides, different pressure cookers, different retrieval
solution and even different ER antibodies. One from Ventana and one from
Zymed. If you can help, or give me a few thing to try I would appreciate


Histonet mailing list

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information.

Histonet mailing list

<< Previous Message | Next Message >>