RE: [Histonet] Destaining DifQuik[Scanned]

From:Kemlo Rogerson

Oops sorry, you already said it! That's the trouble being in the UK, life
has passed you by as you sleep.

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194

-----Original Message-----
From: Tony Henwood [] 
Sent: 02 December 2004 22:46
To: 'Mary North';
Subject: RE: [Histonet] Destaining DifQuik[Scanned]

To destain Romanowsky type stains, place in 1% acetic acid in 90% ethanol.

If the smears have been air-dried prior to DifQuik staining, then PAP
staining will be very dissapointing since the cells will be affected by
air-drying. There is no technique known to reverse this.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Mary North [] 
Sent: Friday, 3 December 2004 3:40 AM
Subject: [Histonet] Destaining DifQuik

Has anyone tried to destain DifQuik-stained cytology preps so that it
can be restained with PAP?
Mary North, HT(ASCP), HTL
Portland, OR
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