RE: [Histonet] CD34 and CD31 in paraffin mouse tissue

From:"Elizabeth Chlipala"

Michelle

It also works on rat.  I have tried it on formalin perfused rat brains
and it works.  I use steam retrieval for 20 minutes, antibody at 1:500,
30 minutes RT, a rabbit anti-goat from vector at 1:400 and then rabbit
envision from Dako to amplify.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: Michelle McDonald [mailto:MichelM9@chw.edu.au] 
Sent: Wednesday, December 15, 2004 2:06 PM
To: 'Elizabeth Chlipala'; wasielewski.reinhard.von@mh-hannover.de;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CD34 and CD31 in paraffin mouse tissue

Dear Liz,

Would you know whether this antibody from Santa Cruz would work on rat
tissue. I am currently trying to get a CD31 antibody from Calbiochem to
work
on FFPE rat bone sections (decalcified) and I am having no luck. Can you
suggest an antibody I could try or recommend an antigen retrieval
technique.
I have tried Proteinase K and Pepsin.

Thank you in advance for your advice

Michelle 

Michelle McDonald B.MedSci
 
PhD Student
Research Assistant
Orthopaedic Research Dept.
The Children's Hospital Westmead.
Westmead NSW 2145
Australia
 
Ph. +612 9845 3087
Fax. +612 9845 3078
 


-----Original Message-----
From: Elizabeth Chlipala [mailto:liz@premierlab.com] 
Sent: Thursday, 16 December 2004 2:52 AM
To: wasielewski.reinhard.von@mh-hannover.de;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CD34 and CD31 in paraffin mouse tissue


Reinhard

Santa Cruz has a goat polyclonal for CD-31 (PECAM-1) that will work in
FFPE
tissue with antigen retrieval. Cat SC-1056.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
wasielewski.reinhard.von@mh-hannover.de
Sent: Wednesday, December 15, 2004 7:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD34 and CD31 in paraffin mouse tissue

Hello Histonetters,
we are looking to immunostain endothelial cells (vessels) in FFPE mouse
tissue. 
There is no cross-reactivity to the human epitopes, therefore the
antibodies
for 
human tissue don't work (we have tried it). Is there anyone out there
who
can help us 
? (Clones, suppliers, ...)
Answers wanted :-)

Best wishes for the rest of this year

Reinhard von Wasielewski


PD Dr. med. Reinhard von Wasielewski


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>