We use a rat anti mouse CD31 from BD Biosciences that works nicely at 1:50 - 1:100 with 8 minutes proteinase K digestion.
	 
	Cheers,
	 
	Jason
	 
	 
	Jason Palmer 
	Bernard O'Brien Institute of Microsurgery 
	42 Fitzroy St, Fitzroy Victoria 3065 
	Australia 
	tel +61 3 9288 4018 
	fax +61 3 9416 0926 
	email: palmerj@svhm.org.au 


	Date: Wed, 15 Dec 2004 15:49:37 +0100 
	From: wasielewski.reinhard.von@mh-hannover.de 
	Subject: [Histonet] CD34 and CD31 in paraffin mouse tissue 
	To: histonet@lists.utsouthwestern.edu 
	Message-ID: <41C05D11.15565.2FFF9314@localhost> 
	Content-Type: text/plain; charset=US-ASCII 

	Hello Histonetters, 
	we are looking to immunostain endothelial cells (vessels) in FFPE mouse tissue. 
	There is no cross-reactivity to the human epitopes, therefore the antibodies for 
	human tissue don't work (we have tried it). Is there anyone out there who can help us 
	? (Clones, suppliers, ...) 
	Answers wanted :-) 

	Best wishes for the rest of this year 

	Reinhard von Wasielewski 


	PD Dr. med. Reinhard von Wasielewski