[Histonet] Re: Cryoembedding media woes, some advice and not a bash session
I appreciate your advice. I always read with great interest your notes on Histonet. Galina Deyneko
Gayle Callis wrote:
I am not bashing anyone's cryomedia, but there are caveats about trying out
new products without testing them out before the BIG project comes
along. No matter which cryoembedding media you desire to use, one should
ask for a sample or purchase the new product before filling lumens, lungs,
small intestines to test the media holding quality before embarking on a
whole, gigantic study.
We tested a new cryoembedding media with great hopes it would work for
us. We use cryoembedding media to fill/distend intestinal lumen to
visualize villi and Peyer's patches. We found the new media did NOT
support the villi. We normally use OCT for this purpose, and found the OCT
supported these fine tissue structures, allowing perfect sections with high
quality morphology. The other new media did not work, sections were
compressed, villi were chewed up and section quality was poor. It was
later found the newer media works great with human tissues and was never
tested on murine or animal tissue. I have no doubt the cryomedia was
excellent for human work, but failed to work for murine special needs
Advice it to test whatever is on the market as one or more cryoembedding
media will show up that is superior for some things we do in the name of
If you find the embedding media is excellent, better holding power - let us
know - the info will be appreciated.
At 11:36 AM 12/8/2004, you wrote:
>Hi Dr.Scouten and histonetters,
>Does it mean that Cryo M Bed more "supportive" than OCT. I work with
>murine carotid arteries with atheroscl. plaque. .I fix them for 2 hours
>in 10 % formalin and embed in OCT, but I have to cut with T -28 C in
>microtome and mount on cool slides , otherwise I am not able to obtain
>good vessel' lumen and plaque scrolls up or falls down and still I
>receive 30% of distorted slices. Any advices will be appreciate.
>Novartis Cambridge MA
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Veterinary Molecular Biology
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