[Histonet] RE: fixation for RE: Masson's Trichrome on frozen heart sections
After sectioning we put the slide in a coplin jar with alcoholic formalin for 5 minutes, and proceed with the stain.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
My opinions are my own and do not reflect those of my employer. Long live free speech!
From: Gayle Callis [mailto:firstname.lastname@example.org]
Sent: Thursday, December 02, 2004 11:20 AM
To: GUTIERREZ, JUAN; Histonet@lists.utsouthwestern.edu
Subject: fixation for RE: Masson's Trichrome on frozen heart sections
How do you fix fresh tissue frozen sections? Bouins or NBF? and fixation
times? If we were to do this stain, our tissues are NEVER prefixed before
A one step staining procedure makes a lot of sense when handling more
fragile frozen sections. I know one substitute light green with aniline
blue, or vice versa - what ever one likes best. This was discussed just a
few months ago on Histonet.
At 09:48 AM 12/2/2004, you wrote:
>We use Gomori's trichrome on frozen sections. It's a one step procedure
>that gives you a faster answer. Are you able to try alternatives to MTS?
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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