[Histonet] RE: Cryo Mbed

From:"Charles Scouten"

Yes, Cryo M Bed is firmer and more supportive.  Try to inject some of
whatever you use into the lumen before freezing.  That would help
greatly.  Call me off histonet and we will see if we can arrange a


Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 




From: Galina Deyneko [mailto:galinadeyneko@yahoo.com] 
Sent: Wednesday, December 08, 2004 12:37 PM
To: histonet@lists.utsouthwestern.edu
Cc: Charles Scouten
Subject: Cryo Mbed

Hi Dr.Scouten and histonetters,
Does it mean that Cryo M Bed more "supportive" than OCT. I work with 
murine carotid arteries with atheroscl. plaque. .I fix them for 2 hours 
in 10 % formalin and embed in OCT, but I have to cut with T  -28 C in 
microtome and mount on cool slides , otherwise I am not able to obtain 
good  vessel' lumen and plaque scrolls up or falls down and still I 
receive 30% of distorted slices. Any advices will be appreciate.
Galina Deyneko
CV Department
Novartis Cambridge MA


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