[Histonet] IHC queries Bowman's layer and Descemet's Membrane
I have been doing ophthalmic histology for 25 years. You do not need to do an immuno to demonstrate Descemet's membrane in the cornea. The simple PAS (periodic acid - Shiff's) stain will stain Descemet's a bright hot pink. It is routinely done with an H&E on recipient corneas from transplants. Bowman's, however, will not stain with PAS, but is the homogeneous layer right beneath the epithelium.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway
Moore, OK 73160
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