[Histonet] IHC on frozen brain ... help
I am assuming you are sectioning mouse brain.
What animal is your primary antibody made in? It should not be mouse, but any other species.
Your primary should not be made in the same species your test tissue is from. UNLESS, you use a mouse-on-mouse kit for mouse tissue.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway
Moore, OK 73160
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