[Histonet] IHC ~ Tissue Falling Off Slides, drying procedure.slides
Hey Jeff! We have tried many variations on drying as well. Generally, we
cut the tissue, let it stand and drain/air dry at room temp for 0.5-1.0
hour, then place on the slide dryer at 65-70 for another hour, then place in
the slide oven at 80 to melt - usually about 20 minutes.
We have also tried microwave drying.
We have also dipped the slides (after the tissue is on them) into
Poly-L-Lysine.
Even slides that are cut on Saturday, dry all weekend and not retrieved
until Monday morning are troublesome.
Dottie - we happen to have a few boxes of Superfrost Plus slides here from
our Cytology department and are cutting some test slides as I type this.
Thanks for the suggestion!
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