[Histonet] ER staining problem

From:"Chris Evanish"

I need your help.
I use a Ventana ES and am having a real problem getting the ER antibody to work. 
It was always a great result, however in the last 6 months or so it has gotten worse to the point now where the stain is dead negative.
I've tried different slides, different pressure cookers, different retrieval solution and even different ER antibodies. One from Ventana and one from Zymed.
If you can help, or give me a few thing to try I would appreciate it.


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