FW: [Histonet] Agitation of cassettes

From:"DiCarlo, Margaret"



Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
100 High St.
Buffalo, NY  14203
716-859-1293
 


-----Original Message-----
From: DiCarlo, Margaret 
Sent: Friday, November 12, 2004 15:20
To: 'Patsy Ruegg'
Subject: RE: [Histonet] Agitation of cassettes


Patsy,

I too also process most of my bone manually.  I agitate on a magnetic stir
plate at room temperature with the bone sitting on a piece of plastic or
something of that nature. I never used it for fixation. I just usually let
it sit in formalin for 5-7 days depending on size and thickness. How much
less time are we talking about, with a bone section that is around 5-8mm in
thickness?  

Thanks for your response.

Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
100 High St.
Buffalo, NY  14203
716-859-1293
 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy
Ruegg
Sent: Friday, November 12, 2004 12:36
To: 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Agitation of cassettes


I too have always processed most of my tissue by hand (usually bone) and
swear by using a shaker for all steps, fixation, decal, etc.  For mass
processing using a tissue processor mine has a stir bar at the bottom of
the tank and I do choose vacuum when ever possible.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Molinari, Betsy
Sent: Friday, November 12, 2004 6:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Agitation of cassettes


Hi Fran,
Happy Friday to you! We do put our cassettes on a shaker. I work in a
research lab and have the luxury of time (well, most of the time I do)
to do so. I usually just keep the cassettes on overnight. I always put
my decals on a rotator or shaker it cuts my decal time by quite a bit. I
do not have a publication, but I am someone on this site will! Betsy
Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology
Houston,TX 77030 832-355-6524


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fran
Lemons
Sent: Thursday, November 11, 2004 2:20 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Agitation of cassettes)

Hello all, it's almost Friday!!

Has anyone out there ever heard of placing the basket of cassettes in
formalin on an agitator to stir the formalin as a way to expedite
fixation?  If so, can you direct me to something published on the
subject? Also, has anyone heard of the same thing for bunions in decal
solution? Thanks ahead of time, Fran Walker


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