I agree with Bonnie about preheating the HIER buffer – quick & easy. Also, we prefer the oval shaped steamer over the round one. The oval one holds more water. We do fill it up to the brim!

Patti Loykasek
PhenoPath Laboratories
Seattle, WA


I use the B&D too, and I just fill the steamer fuller than you are supposed to..... almost to the top, and it always has enough for preheating and the longest time I use (40 min).  The other thing I do sometimes (more for speed than for the reason you mention) is to heat the buffer in the microwave for a bit first.  Then the preheating time is less than 20 minutes.
Bonnie Whitaker
University of Texas Medical School at Houston
6431 Fannin Street
MSB 2.231
Houston, Texas  77030
Phone 713.500.6792
Fax 713.500.0733

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
Sent: Wednesday, December 17, 2003 12:13 PM
To: Kim Merriam; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] steamers

Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends to run out of water if not topped up - it takes about 15-20 min to bring the temp of citrate buffer in a Coplin jar up to 95 deg C.

Min-han Tan

 

-----Original Message-----
From: Kim Merriam [mailto:kmerriam2003@yahoo.com]
Sent: Wednesday, December 17, 2003 12:04 PM
To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] steamers

I just use a Black & Decker Flavor Scenter Handy Steamer.  About 30 bucks at Walmart!
 
Kim Merriam
Novartis
Cambridge, MA

"Bartlett, Jeanine" <jqb7@cdc.gov> wrote:

All:

Can anyone recommend a good, laboratory-grade steamer for antigen retrieval?  

Thanks!
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333

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