Re: [Histonet] mouse bile duct epithelium
I have used a rat monoclonal against mouse cytokeratin CK8 (clone TROMA-1;
from U of Iowa Hybridoma Bank). It stained well, but at the dilution
recommended (1:2) I felt there was nonspecific background as well. With
more of a work-up, this could probably be improved.
In the following article you will find other mouse CKs listed and their
sources- Spontaneous mouse mammary tumors: incidence and cytokeratin
expression; Research in Veterinary Science; 1997; 63; 85-89.
U of MN Vet Diag Lab
----- Original Message -----
From: "Patsy Ruegg"
To: "Histonet@Pathology. Swmed. Edu"
Sent: Thursday, January 08, 2004 2:24 PM
Subject: [Histonet] mouse bile duct epithelium
> here is my story:
> i am trying to label ms bile duct epithelium with mouse anti-mouse
> cytokeratin 7 and 19 (sounds odd, but that is what the investigator tells
> it is, not even rat anti-mouse) they tried the anti-human ck7&19 and it
> not cross react with the mouse. I have the reagents to biotinylate these
> ms biotin is a huge problem even with the most aggressive A/B blocking. Is
> there another possible rabbit antibody I could use that would label ms
> duct epithelial cells? Would factor VIII, cd31, or cd34 stain these
> I know that the previous mentioned markers are for endothelial cells but I
> am just hoping they might work????? I have not been able to find any
> say rabbit anti-ms ck7 or ck19 out there, has anyone else. How about the
> new rabbit monoclonals, is anyone making ck7/ck19? By the way I have
> and ffpe bile duct samples, so I can use either.
> Please advise.
> Patsy Ruegg
> Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>