Re: [Histonet] Tissue Separating from paraffin ribbon

From:Gayle Callis

You didn't say how you fixed, time, what animal or processing schedule and
if you use vacuum/pressure, etc.   2 hours per change of paraffin may be
excessive for lean aka less fatty animal tissues.  Dirty paraffin is more
likely to cause ski tracks in the sections.  

Do you ice water soak your tissues after trimming for a short time?  

Check your paraplast plus, we used to get water droplets in bottom of this
paraffin after melting, strange - even though we stored the paraffin

 At 07:33 AM 1/8/2004 -0500, you wrote:
>We are experiencing our animal tissue separating and rolling back on itself 
>at sectioning.
>We currently use Kendall's Paraplast plus and we infiltrate in two changes 
>of paraffin at processing each being two hours.  We use the open auto 
>technicon processors.  The processors were changed prior to processing so we 
>do not believe the paraffin was dirty.  Any insight would be greatly 
>Thank you,
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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