Hi Mike

We've have a similar problem on occasions with Surgipath slides as well. We also have the DAKO Autostainer and use DAKO diluent and detection reagents.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England

>  from:    Mike Bromley 
>  date:    Tue, 23 Dec 2003 11:22:07
>  to:      scoticc@bigfoot.com, dodson@liv.ac.uk, histonet@lists.utsouthwestern.edu, suzy.howard@dakocytomation.co.uk, katrina.murphy@dakocytomation.co.uk
>  subject: Re: [Histonet] Superfrost plus slides
> 
> Hi All
> 
> We have had a problem with a batch of Menzel Superfrost Plus slides
> (3451272). They have been made in a way that makes them more hydrophobic
> than usual.
> 
> The problem may not have been noticed by others because it only occurs under
> certain circumstances, however the consequences can be extremely serious.
> 
> The circumstances are:
> 1.	Use of a "flatbed" stainer where the slides are held horizontally
> 2.	Use of a bad batch of slides
> 3.	Use of these slides for antibodies which do not require pressure
> cooking, this usually means enzyme treatment or no treatment (sometimes even
> when heat treated in a 95-99* water bath as in the Herceptest) Pressure
> cooking the slides seems to overcome the hydrophobic nature of the "bad"
> slides.
> 
> Wash buffer usually spreads okay on the slides, but when the reagent is
> dripped onto the slide it fails to spread evenly. This is usually not a
> problem if the section is a single piece of tissue and it is hit by the
> drops however if the section is composed of small pieces then some of these
> can be missed. Hence your controls may be fine, because they are often a
> single piece of a reasonable size, but you may miss a critical piece of
> tissue.
> 
> I advise everyone who uses a flatbed horizontal stainer for sections not
> requiring pressure cook antigen retrieval to watch the addition of the
> dropped reagents. This problem is due to batch variation and may be slight
> or extreme depending on the batch.
> 
> Other users have noted problems with Superfrost Plus slides before (see
> histonet archives) We use Superfrost Plus slides because they are better
> than home made APES slides especially for our slides that are pressure
> cooked in high pH solution.
> 
> All our reagents are from Dako and the Autostainer protocol is followed.
> 
> Best Wishes
> 
> Mike Bromley
> 
> Chief Biomedical Scientist
> Pathology
> Dumfries & Galloway Royal Infirmary
> Scotland, UK
> 
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