Re: [Histonet] Iron Stain on Bone Marrow Smears

blown out nuclei iss not a stain problem , but has been a problem in our lab from temp and believe it or not exhausted xylene, i say this because you will notice that this is 
1. an intermitent problem
2. may occur (as it did with us) on any number of different stains
3. tends to show up later in the week(when reagents are getting older)

my suggestion to you is to change your depapraffinizing station mor often or use only fresh reagents for bone marrows and see if it still occurs. we now change our xylenes more often and did reduce temp and we very rarely if almost never
see this problem.    good luck  dana

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