From: | "Patsy Ruegg" |
I use the
steamer and heat the ar buffer in the mw to boiling before putting it in the
steamer that way it only takes about 5 min. to preheat.
Patsy
-----Original
Message-----
From:
histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
Sent: Wednesday, December 17, 2003
11:13 AM
To: Kim Merriam; Bartlett,
Jeanine; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] steamers
Yes, we use that too.
Only problem is that for long ARs (30-35 min), it tends to run out of
water if not topped up - it takes about 15-20 min to bring the temp of citrate
buffer in a Coplin jar up to 95 deg C.
Min-han Tan
-----Original Message-----
From: Kim Merriam
[mailto:kmerriam2003@yahoo.com]
Sent: Wednesday, December 17, 2003
12:04 PM
To: Bartlett, Jeanine;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] steamers
I just use a Black & Decker Flavor
Scenter Handy Steamer. About 30 bucks at Walmart!
Kim Merriam
Novartis
Cambridge, MA
"Bartlett,
Jeanine" <jqb7@cdc.gov> wrote:
All:
Can
anyone recommend a good, laboratory-grade steamer for antigen retrieval?
Thanks!
Jeanine
Bartlett, HT(ASCP)
Centers
for Disease Control
Infectious
Disease Pathology Activity
1600
Clifton Road, MS/G-32
Atlanta,
GA 30333
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