RE: [Histonet] steamers

From:"Patsy Ruegg"

Message

I use the steamer and heat the ar buffer in the mw to boiling before putting it in the steamer that way it only takes about 5 min. to preheat.

Patsy

 

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan
Sent: Wednesday, December 17, 2003 11:13 AM
To: Kim Merriam; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] steamers

 

Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends to run out of water if not topped up - it takes about 15-20 min to bring the temp of citrate buffer in a Coplin jar up to 95 deg C.

 

Min-han Tan

 

 

-----Original Message-----
From: Kim Merriam [mailto:kmerriam2003@yahoo.com]
Sent: Wednesday, December 17, 2003 12:04 PM
To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] steamers

I just use a Black & Decker Flavor Scenter Handy Steamer.  About 30 bucks at Walmart!

 

Kim Merriam

Novartis

Cambridge, MA

"Bartlett, Jeanine" <jqb7@cdc.gov> wrote:

All:

 

Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? 

 

Thanks!

Jeanine Bartlett, HT(ASCP)

Centers for Disease Control

Infectious Disease Pathology Activity

1600 Clifton Road, MS/G-32

Atlanta, GA 30333


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