RE: [Histonet] folds forming in bone/cartilage sections

From:"Elizabeth Chlipala"


We place all of our bone sections on a hot plate (around 40 degrees C,
the paraffin should not melt) overnight and then stain them.  If you
need to take a look at some images you can go to our web page, there
should be some images in the gallery on mouse joints, and a few pdf
files that you could down load that might be helpful.


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
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-----Original Message-----
[] On Behalf Of Mike
Sent: Wednesday, January 14, 2004 11:37 AM
Subject: [Histonet] folds forming in bone/cartilage sections


	I find that, when sectioning EDTA-decalcified mouse tibiae at 
5 - 10 microns, that folds (i.e., creases) frequently form 
specifically within the growth plate cartilage as the sections dry 
and adhere to the Superfrost slides.  I have had no success with 
increasing the amount of time that I float the sections on a water 
bath prior to drying them on slides.  I'm hoping that someone might 
be able to offer a suggestion as to how I can overcome this 
difficulty.  Thanks very much.


Michael Sohaskey, Ph.D.
University of California, Berkeley
Molecular and Cell Biology
585 Life Sciences Addition
Berkeley, CA  94720-3200
(510) 643-2775 (phone)

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