RE: [Histonet] Superfrost plus slides

From:"Bill Sinai"

Merry Christmas to all from down under in Australia.

We to have had problems but only with limited numbers of slides.  We were
told that there was "a" batch of Superfrost Slides which were suspect.  I
found out that nearly all Superfrost Slides are manufactured in one or two
factories in Germany and packaged for the local distributors.  The company
claimed they knew nothing about problems with any of their slides, however
there were two batches which gave problems one was # 3421272 and I believe
the other also ended in 72.

This happened about 3-4 months ago now and I have noticed that all the
batches have expiry dates on them.

>From correspondence on Histonet it appears that the problem or something
like it has been going on for several months.

But as is usual in Histotechnology everyone tries to solve the problem
in-house without discussing it with anyone else.

This is where I find Histonet invaluable.

Bill Sinai
Laboratory Manager
Tissue Pathology, IMPAR
Westmead NSW 2145
Ph 02 9845 7774

-----Original Message-----
[]On Behalf Of
Sent: Wednesday, 24 December 2003 12:33 AM
Subject: Re: [Histonet] Superfrost plus slides

Hi Mike

We've have a similar problem on occasions with Surgipath slides as well. We
also have the DAKO Autostainer and use DAKO diluent and detection reagents.

Nick Kirk
Hinchingbrooke Hospital

>  from:    Mike Bromley 
>  date:    Tue, 23 Dec 2003 11:22:07
>  to:,,,,
>  subject: Re: [Histonet] Superfrost plus slides
> Hi All
> We have had a problem with a batch of Menzel Superfrost Plus slides
> (3451272). They have been made in a way that makes them more hydrophobic
> than usual.
> The problem may not have been noticed by others because it only occurs
> certain circumstances, however the consequences can be extremely serious.
> The circumstances are:
> 1.	Use of a "flatbed" stainer where the slides are held horizontally
> 2.	Use of a bad batch of slides
> 3.	Use of these slides for antibodies which do not require pressure
> cooking, this usually means enzyme treatment or no treatment (sometimes
> when heat treated in a 95-99* water bath as in the Herceptest) Pressure
> cooking the slides seems to overcome the hydrophobic nature of the "bad"
> slides.
> Wash buffer usually spreads okay on the slides, but when the reagent is
> dripped onto the slide it fails to spread evenly. This is usually not a
> problem if the section is a single piece of tissue and it is hit by the
> drops however if the section is composed of small pieces then some of
> can be missed. Hence your controls may be fine, because they are often a
> single piece of a reasonable size, but you may miss a critical piece of
> tissue.
> I advise everyone who uses a flatbed horizontal stainer for sections not
> requiring pressure cook antigen retrieval to watch the addition of the
> dropped reagents. This problem is due to batch variation and may be slight
> or extreme depending on the batch.
> Other users have noted problems with Superfrost Plus slides before (see
> histonet archives) We use Superfrost Plus slides because they are better
> than home made APES slides especially for our slides that are pressure
> cooked in high pH solution.
> All our reagents are from Dako and the Autostainer protocol is followed.
> Best Wishes
> Mike Bromley
> Chief Biomedical Scientist
> Pathology
> Dumfries & Galloway Royal Infirmary
> Scotland, UK
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