We use Dako's antibodies with a steamer.  They are beautiful.  We no longer
use the pressure cooker.

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio



-----Original Message-----
From: Anissa Choi [mailto:anissachoi@hotmail.com] 
Sent: Tuesday, December 16, 2003 10:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re : Kappa and Lambda


Does anyone pressure cook the paraffin sections before  immunostaining for 
Kappa and Lambda?
Our regular protocols : no pretreatment for B5, Protease for buffered 
formalin. I have found that
there are a lot more plasma cells get stained after pressure cooking but 
backround is heavy though.

Thanks in advance for your input.

Anissa Choi
Burnaby Hospital
Burnaby, B.C.
Canada

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