RE: [Histonet] PGP 9.5

From:"Mitchell (Jean A.)"

I have been using a polyclonal PGP 9.5 for immunolabeling epidermal
neurites on 50um frozen sections of skin. Are you staining sections on
slides or free floating your sections in well plates?  I have attempted
both and by far the most consistant results are obtained by free
floating the sections in well culture plates for the entire staining

Good luck & contact me directly if I can assist you more with this

Jean Mitchell, BS, HT (ASCP)
University of Wisconsin Hospital & Clinics
Department of Neurology
Madison, WI

-----Original Message-----
From: Chow Wai Lyn, Adeline [] 
Sent: Tuesday, January 13, 2004 9:53 PM
Subject: [Histonet] PGP 9.5

Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin
taken from the hypothenar region. The results have been inconsistent so
far , sometimes staining well, sometimes not. Sections are 30um and
stained overnight room temp. using DAKO envision kit. Any advice ?

Research Assistant
National University of Singapore

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