I have had very good results staining nerve fibers with Kiernan's physically
developed silver method [Kiernan, HISTOLOGICAL AND HISTOCHEMICAL METHODS,
3rd ed., pp371-373].

Allen A. Smith, Ph.D.
Professor of Anatomy
School of Graduate Medical Sciences 
Barry University 
Miami Shores, FL

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chow Wai
Lyn, Adeline
Sent: Tuesday, January 13, 2004 10:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PGP 9.5


Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin taken
from the hypothenar region. The results have been inconsistent so far ,
sometimes staining well, sometimes not. Sections are 30um and stained
overnight room temp. using DAKO envision kit. Any advice ? Thanks..

Lyn
Research Assistant
National University of Singapore


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Barry University - Miami Shores, FL (http://www.barry.edu) 

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