RE: [Histonet] Osteoclast stain

From:"Gehan, Loralee"

I do a TRAP stain, the enzyme histochemical way just about everyday and all
of my samples are fixed in formalin.  For the plastic sections (haven't been
done in some time)  we fixed in an alcoholic formalin.  
I can send a protocol if needed.  It's not a kit but it is very similar.
The antibody I have search high and low for and all I can find is one that
works wonderfully in human tissue, not in animal.  
Hope that helps a little.   
Loralee Gehan
University of Rochester
Orthopaedics Research Lab

> ----------
> From: 	Patsy Ruegg
> Sent: 	Tuesday, January 13, 2004 12:25 PM
> To: 	Paul Keogh; 'Histonet (E-mail)
> Subject: 	RE: [Histonet] Osteoclast stain
> 
> Paul,
> If you have not fixed with formalin you could do Acid Phosphatase (TRAP)
> for
> osteoclasts, this is the enzyme histochemical method.  I can provide you
> the
> method if you like.  I used a kit from Sigma, I hope someone is still
> selling it.  There is also an antibody but I do not have experience with
> it.
> Patsy
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Paul
> Keogh
> Sent: Tuesday, January 13, 2004 5:33 AM
> To: 'Histonet (E-mail)
> Subject: [Histonet] Osteoclast stain
> 
> 
> 
> 
> I have sections of   MMA embedded rat femur . I want to stain for
> osteoclasts.
> 
> Can anyone recommend a tried and tested protocol please.?
> 
> Paul
> 
> 
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