RE: [Histonet] Osteoclast stain

From:"Patsy Ruegg"

If you have not fixed with formalin you could do Acid Phosphatase (TRAP) for
osteoclasts, this is the enzyme histochemical method.  I can provide you the
method if you like.  I used a kit from Sigma, I hope someone is still
selling it.  There is also an antibody but I do not have experience with it.

-----Original Message-----
[]On Behalf Of Paul
Sent: Tuesday, January 13, 2004 5:33 AM
To: 'Histonet (E-mail)
Subject: [Histonet] Osteoclast stain

I have sections of   MMA embedded rat femur . I want to stain for

Can anyone recommend a tried and tested protocol please.?


This email is confidential and intended solely for the use
of the individual to whom it is addressed.
 Any views or opinions presented are solely those
of the author and do not necessarily represent those
of the Dublin Dental School and Hospital.  If you are not
the intended recipient, be advised that you have
received this email in error and that any use,
dissemination, forwarding, printing or copying of this
email is strictly prohibited.  If you have received this
email in error, please contact the sender.

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>