[Histonet] stain for osteoblasts

From:John Baker

Does anyone have a protocol for something like this?

Specimen cells are fixed in 10% aqueous formaldehyde, serially 
dehydrated, the specimens are embedded in paraffin and cut into 5um 
thick cross sections for H&E or von Kossa silver nitrate/alizarin red 

The question is if anyone has a more detailed protocol for staining
for bone formation with osteoblasts fixed inside a polymer (alginate in
this case).

The protocol is wanted by a graduate student in our lab and I am 
unfamiliar with working with cells.
Thank you for any assistance with this! John


Today is the Tomorrow you worried about Yesterday. Was
it worth it?

Birthdays are good for you. Statistics show that the
people who have the most live the longest.

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635

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