[Histonet] proteins in vesicles staining

From:am102@st-andrews.ac.uk (Anne-Sophie Martinez)

Hello everybody!!!

I want to stain in IHC a protein (guanylin) which is supposed to be in
intra-cellular vesicles in the epithelium. Could somebody let me know (1)
if there is a protocol (and which one) to premeabilize these vesicles to
allow the AB to fix on the protein inside? or (2) if something better does

Thank you very much.
Merry Christmas.


Dr Anne-Sophie Martinez
School of Biology
University of St. Andrews
Bute Medical Buildings
St. Andrews
Fife, KY16 9TS

Tel + 44 (0) 1334 463546
Fax + 44 (0) 1334 463600

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