[Histonet] freezing mouse lungs

From:Rita Angel

Hi all,

I was wondering if anyone could offer help in obtaining the best sections 
possible with frozen mouse lungs. How can I prepare the lungs and freeze 
them to get the best results? I know this is difficult tissue to get good 
sections on anyway, with all the air pockets. Is it possible to infuse them 
with freezing embedding media?

The lung sections I'm getting have a lot of holes, and at best, I'm only 
getting tissue from half of the lung samples while cutting. They were 
brought to me already frozen and embedded so I'm not sure how they were 
prepared.

Thanks for your help,

Rita Angel, HT
University of Cincinnati


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