[Histonet] folds forming in bone/cartilage sections

From:Mike Sohaskey


	I find that, when sectioning EDTA-decalcified mouse tibiae at 
5 - 10 microns, that folds (i.e., creases) frequently form 
specifically within the growth plate cartilage as the sections dry 
and adhere to the Superfrost slides.  I have had no success with 
increasing the amount of time that I float the sections on a water 
bath prior to drying them on slides.  I'm hoping that someone might 
be able to offer a suggestion as to how I can overcome this 
difficulty.  Thanks very much.


Michael Sohaskey, Ph.D.
University of California, Berkeley
Molecular and Cell Biology
585 Life Sciences Addition
Berkeley, CA  94720-3200
(510) 643-2775 (phone)

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