[Histonet] autofluorescence in thraustochytrids (protists)

From:"McCollough, Carol"


I have a question on behalf of a colleague.  She is observing autofluorescence in thraustochytrids fixed in Davidson's AFA.  The paper she is using as a reference for the acriflavine staining technique used 3.7% formaldehyde in seawater with Lugol's iodine (no concentration specified) as the fixative, and autofluorescence did not occur.  Would anyone comment as to whether a difference in fixation would induce autofluorescence?  Does Lugol's iodine quench autofluorescence?

Thanks for your insights.

Regards -
Carol B. McCollough, HT/HTL (ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, Maryland 21654

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