[Histonet] autofluorescence in thraustochytrids (protists)
Greetings:
I have a question on behalf of a colleague. She is observing autofluorescence in thraustochytrids fixed in Davidson's AFA. The paper she is using as a reference for the acriflavine staining technique used 3.7% formaldehyde in seawater with Lugol's iodine (no concentration specified) as the fixative, and autofluorescence did not occur. Would anyone comment as to whether a difference in fixation would induce autofluorescence? Does Lugol's iodine quench autofluorescence?
Thanks for your insights.
Regards -
Carol
******************
Carol B. McCollough, HT/HTL (ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, Maryland 21654
cmccollough@dnr.state.md.us
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>