[Histonet] autofluorescence in thraustochytrids (protists)
I have a question on behalf of a colleague. She is observing autofluorescence in thraustochytrids fixed in Davidson's AFA. The paper she is using as a reference for the acriflavine staining technique used 3.7% formaldehyde in seawater with Lugol's iodine (no concentration specified) as the fixative, and autofluorescence did not occur. Would anyone comment as to whether a difference in fixation would induce autofluorescence? Does Lugol's iodine quench autofluorescence?
Thanks for your insights.
Carol B. McCollough, HT/HTL (ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, Maryland 21654
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