From: | "George Cole" |
For Those Who Received Muscle & Nerve packets;
Sorry, when I made up the packets, I used forms filled out
for our last inspection by office staff.
The typing was so much better than my own. All the ingredients were listed, but the
actual PAS procedure was left out.
The following procedure is done after the PAS reagents are
prepared:
This follows page 12:
1. Put slides in the fixative 10 minutes to one hour.
2. Rinse well in deionized water.
3. Place in freshly mixed 0.5% aqueous Periodic Acid 5 minutes.
4. Wash thoroughly in deionized
water.
5. Place in Schiff’s Reagent 15 minutes.
6. Rinse in three changes of acid rinse for two
minutes each.
7. Wash in
gently running water
for a minute or two. Rinse
with deionized water. Stain with Gill’s III hematoxylin
for
30 seconds to a
minute. Blue and rinse with running
tap water 15 minutes. Rinse in deionized
water.
8 . Dehydrate
with 95% and 100% alcohols. Clear
in xylene and mount in Permount
or equivalent.
DIASTASE DIGESTION PROCEDURE:
1. Fix for 10 minutes.
2. Rinse well.
3. Digest in filtered Diastase solution for one hour at 37C.
4. Rinse well in
deionized water.
5 Proceed with regular PAS routine from step 3 above.