Hi,all
Thanks for your kindly responses.
My second Ab is said to be a mAb in the description file from manufacture. But do not say if it was absorbed by rat IgG, problely not.
David Wright is right. There is an inflammatory reaction as seen by mononuclear cell infiltration and diapedesis in the tissue.
So maybe add rat serum to blocking and antibody diluent reagent will work. I will try this out. 

				

　　　　　　　　Baowei Peng
　　　　　　　　pengbaowei@21cn.com
　　　　　　　　　　2003-12-30



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet