[Histonet] Frozen section ice artifact
From: | Jackie.O'Connor@abbott.com |
Yo Histonetters, and Happy New Year.
I just cut some frozen sections of solid tumors in OCT which were supposed
to be snap frozen in liquid nitrogen after being put in plastic cryomolds.
I have never seen such horrible morphology in my career. I think this is
an example of the absolute worst ice crystal artifact I have ever seen,
but since I have never seen anything this bad before, I don't know what
I'm seeing, you see? I hope I've intrigued someone enough to email me
directly, so I can send you a gif file of this atrocity. I think the
tissues were mishandled prior to freezing (someone else froze them) and
I'm trying to figure out what happened so I can correct the problem. I'd
appreciate anyone who would like to take a look at this artifact - it's
awful, honest!
Jackie O'Connor
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