Re: [Histonet] mouse microglia
Hi Inga:
"Mac1" or "F4/80" will stain microglia in frozen sections of mouse
brain. Fix by perfusion with buffered paraformaldehyde, remove the brain
and fix for another 2 hours, no longer (at least for Mac1). Then rinse
multiple times in buffer, cyroprotect with sucrose, cut 20 micron frozen
sections, store in PBS, stain free-floating, etc. I have stored sections
in PBS in the refrigerator for several weeks with no loss of
antigenicity. Sections air-dired on slides lose microglial antigenicity
very quickly (2 days). Antibodies for rat microglia will not work on mouse.
Clint Lincoln has stained mouse microglia in FFPE sections, he used
a enzyme digestion protocol (pronase?) to unmask the antigenic sites. I
don't know if I can find his recipe.
See: Lawson et al., Neuroscience 39:151-170, 1990 and 48:405-415,
1992 and Perry et al., Neuroscience 15:315-326, 1985.
Geoff
Inga Hansson wrote:
> Hi all!
>
> Can anyone suggest a good antibody for microglia that works on frozen
> and FFPE-tissue in mice?
>
> Thanks in advance!
>
> Inga
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>