Re: [Histonet] microglia

From:Sharon Cooperman

Hi.  I don't think there are any that work well in FFPE.  Abcam has 
one that requires proteinasing antigen retrieval that hasn't worked 
for me.  MAC-1 works well in frozen tissue.  There's also a special 
fixation protocol (prior to paraffin embedding) that is supposed to 
make all the abs that work on frozen tissue work on paraffin embedded 
tissue - the reference is on the abcam site with their 
anti-microglial antibody.  I know f4/80 works for peripheral 
macrophages in ffpe tissue, but I don't know if it works on 
microglia.  Another solution would be to use ricin (not the very 
toxic variety) from Vector (references and protocol on their web 
site.  I would love to find an anti-microglial antibody that is easy 
to use and works of ffpe tissue but I don't think one exists.


>Hi all!
>Can anyone suggest a good antibody for microglia that works on 
>frozen and FFPE-tissue in mice?
>Thanks in advance!
>Inga Hansson
>Dept. neuroscience,
>div. neurobiology
>PO Box 587, BMC
>Husargatan 3
>S-751 23 Uppsala
>Phone: +46(18) 471 4384
>Fax:   +46(18)559017
>Histonet mailing list

Sharon Cooperman       	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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