From: | "Lee & Peggy Wenk" |
----- Original Message -----From: Hernan Aldana MarcosTo: Jill CoxSent: Wednesday, December 03, 2003 1:20 PMSubject: Re: [Histonet] Stain for H.PyloriMODIFICATION OF GIEMSA STAIN FOR HELICOBACTER
FIXATION: Neutral Formolin 10%
SECCIONS: 4-5 µm
SOLUTIONS:
ACETIC WATER
Acetic acid............... 0,01 ml
Destilated water............... 1000,00 ml
Working solution GIEMSA
Giemsa.............. 10,0 ml
Acetic water.............. 50,0 ml
PROCEDDURE
1. Take slides to water destiled
2. Giemsa working solution 1 h
3. Deshidratation in 3 changes of alcohol 100
4. xilol, syntetic mounting medium
RESULTADOS
H. pylorii.............. intense blue
Nuclei.............. blue
Cytoplasm.............. Rose
REFERENCE
Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984Dr. Hernán J. Aldana Marcos
Facultad de Medicina. Universidad de Morón
Machado 914. B1708JPD. Buenos Aires. Argentina
e-mail alternativo hernanjavier@yahoo.com
web: http://hjaldanamarcos.bravepages.com
http://histologia.bigthicketdirectory.net/main.html----- Original Message -----From: Jill CoxSent: Wednesday, December 03, 2003 1:58 PMSubject: [Histonet] Stain for H.PyloriHello everyone,Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance
Jill Cox HT (ASCP)Histology Supervisor
Seattle Histology Lab
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