Re: [Histonet] Joey Wilkie (no subject)

From:Caroline Stott

Have you been filtering the haematoxylin before use?  Also, what haematoxylin are you using?  We use Harris haematoxylin and differentiate the slides in 0.5% acid/alcohol (conc HCL in 70% alc) for 10 seconds.  These steps should eliminate any precipitate.

At 09:30 13/12/03 +1300, you wrote:

We are habing problems with brown precipitant on our slides after we have stained with H&E on our automated stainer.  We do have a Tissue-Tek coverslipper.  The pathologists say the the H&E staining is good.  We called Sukura and they said that maybe our slides were not getting completely dehydrated. We have tried uping the times on our stainer, we are changing the alcohols almost after each rack and we are still getting brown precipitant.  Our pathologist are saying that as they screen the slides that they can see the brown precipitant forming.  When we coverslip by hand we are not getting the brown precipitant.  We can use any and all suggestions that we can get

Thanks, Joey Wilkie, HT
St. Mary's Hospital
Grand Junction, Colorado
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Caroline Stott

Histology Service Unit
Medical School
University of Otago
(03) 479 7152

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