Re: [Histonet] Bone Marrow Biopsy

From:cycling@another.com


Hi Amy

I was wondering whether you may have features of "blue-grey" artefact in your biosies.
If I understand the theory on this, then it is perhaps down to clearing agent being around in the early stages of processing.  
Shandon /ThermoShandon were recommending a warm water wash between processing runs to wash out the pipes on the machines.
There was other advice to make sure that fixation was not compromised, that's to say not too short.
We have been using Neutral buffered formalin, isotonic for saline followed by decal in EDTA.
It is a long time now but I still think the chromatin not quite so condensed as with the old Formic acid decal

Dave Ed'
Christie Hospital
Manchester UK


-----Original Message-----
From : Amy Self 
To : 'histonet@lists.utsouthwestern.edu' 
Date : 05 December 2003 16:38:47
Subject : [Histonet] Bone Marrow Biopsy

>
>	Hello Histonetters !     Hope that everyone had a great
>Thanksgiving.;
>
>	 I was wandering how all of you netters fixed and decaled your bone
>marrow biopsy specimens?  Here lately the doc that reads out our BM cases
>says that the biopsy looks kind of smudgy gray.   What could this be?  He
>wants to believe that it is not getting deparaffinized enough but all of the
>other slides in the rack are just fine..... I am open for any
>suggestions....
>
>Thanks in advance 
>
>	Amy Self
>	Georgetown Memorial Hospital
>	Georgetown,  SC  29440
>
>	843-527-7179 (work)
>	843-520-7882 (fax)
>
>
>
>
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