I've always used graded ethyl alcohol for processing, finishing up with
absolute before going into the clearing agent. Perhaps your times in each
grade were too long, especially the 70% and the absolute.

Lesley Weston.


on 01/12/2003 10:30 AM, Tague, Curtis at CTague@ahs.llumc.edu wrote:

> i remember using some of this stuff many years ago to process tissue and ended
> up with a bunch of fried or burnt looking tissue. i was told ethyl alcohol is
> not good for processing tissue but i think i've heard different since then.
> anyone have any comments? can ethyl alcohol be used to process tissue and the
> error was a result of some other mistake?
> 
> 
> thanks for your help,
> curt
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