Re: [Histonet] 200 proof absolute ethyl alcohol
I've always used graded ethyl alcohol for processing, finishing up with
absolute before going into the clearing agent. Perhaps your times in each
grade were too long, especially the 70% and the absolute.
on 01/12/2003 10:30 AM, Tague, Curtis at CTague@ahs.llumc.edu wrote:
> i remember using some of this stuff many years ago to process tissue and ended
> up with a bunch of fried or burnt looking tissue. i was told ethyl alcohol is
> not good for processing tissue but i think i've heard different since then.
> anyone have any comments? can ethyl alcohol be used to process tissue and the
> error was a result of some other mistake?
> thanks for your help,
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