RE: [Histonet] polymerization problems
Well, this is my 2 cents (South African) worth:
1. I have, as yet, not been able to get the stuff to polymerise at cold
temperatures. I have had very good sucess at RT, although with such large
tissues, trial and error as well as judicious advice from fellow
histonetters, it is recommended that the specimen is "layered" with
embedding solution and allowed to polymerise over a few days. Otherwise
unsightly and potentially bubbly and uncutable blocks result.
2. I have found that, because the 2 embedding solutions are so viscous that
they must be well mixed before use. I generally stir mine for about 20
minutes on a magnetic stirrer before pouring into the mould. (do this in a
3. Heraeus Kulzer have a very nice technical assistant in Germany who has
been very helpful in the past. If all else fails get hold of her for
further insights. She is Ulrike Leins at: Ulrike.Leins@t-online.de
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
----Original Message Follows----
From: "Tom Schaer"
Subject: [Histonet] polymerization problems
Date: Wed, 10 Dec 2003 09:32:05 -0500
I am going to try again, realizing that not many have vast experience with
embedding large specimen with technovit 9100 yet. Maybe someone may be able
to share some pointers for a non-profit lab?
Essentially, we fail to successfully polymerize med fem ovine condyles in
approx 100ml jars. Tried at -15C with no change in solution consistency for
a week; then brought it up to -2C still no change over three days. Jars
were "vacuumed" and set in refrigerator / freezer. I am at the of my rope
Thanks for any pointers.
Thomas P. Schaer, VMD
Assist. Prof. BIOMED, Drexel University
Comparative Orthopaedic Research & Tissue Engineering
Department of Clinical Studies
University of Pennsylvania New Bolton Center
382 West Street Road
Kennett Square, PA 19348
tel. 610-444-5800 (x6261 office)
tel. 610-444-5800 (x6131 lab)
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