RE: [Histonet] The best staining method for new bone formation

From:"Barry R Rittman"

Hi - happy holidays.
I would recommend that you use a trichrome such as Masson's trichrome when osteoid is stained green and bone  red  -  this should allow easy differentiation for image analysis.
Not sure if you are already doing this but it is customary to not only count the thickness of osteoid but also the numbers of osteoblasts, bone lining cells and osteoclasts per unit length.
-----Original Message-----
From: [] On Behalf Of Ronakorn Panjaphongse
Sent: Monday, December 15, 2003 1:14 AM
Subject: [Histonet] The best staining method for new bone formation

Hello everybody
I would like to conduct the study to evaluate the action of Bone Morphogenetic Protein (BMP) antagonist in prevent new bone formation. But the well known H&E stain may be not good enough because both matrix and new bone formation ( osteoid ) is also red, and it is also very hard in quantitative evaluation by using computer program ,eg. NIH too. 
Does anybody has some advice in better staining method or other way in quantitative evaluation that able to differentiate the new bone formation from the peripheral matrix.
Thank you very much for your answer in advance.
Ronakorn Panjaphongse
Graduate Student
Graduate School of Biomedical Science
Hiroshima University

Do you Yahoo!?
New Yahoo! Photos - easier uploading and sharing

<< Previous Message | Next Message >>