RE: [Histonet] Steiner and Steiner method/H. pylori

From:"Bartlett, Jeanine"


We do the Steiner and Steiner regularly.  I used to have issues with our
waste disposal group here about the uranyl nitrate until I showed them
the product insert we received from PolyScientific, which is where we
purchase the product.  It listed all the things that were more
radioactive than the uranyl nitrate solution such as a compass and watch
battery.  I would be glad to send you a copy if it would ease your mind.

As far as the procedure goes, when I have time I use the waterbath
method for the silver as I get less precipitate.  I do use the microwave
for the sensitization step.  It's a nice, clean procedure when done
carefully and gently.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333

-----Original Message-----
[] On Behalf Of Derek
and/or Lynda Leopold
Sent: Tuesday, December 09, 2003 4:30 PM
Subject: [Histonet] Steiner and Steiner method/H. pylori

Hi list,
Would anyone care to comment on the Steiner and Steiner (microwave) 
method for spirochetes?  I susupect my lab's pathologists are interested

in it for viewing H. pylori, yet in all the "HP" talk on this list I 
have yet to hear it mentioned.  How does it compare from a tech safety 
standpoint?  As a brand-new tech, I still do crazy things like read the 
safety warnings on ingredients, and that whole uranyl nitrate 
radioactive thing is sort of bothering me.  We use no protective 
equipment when doing this stain (which the doctors still call "Warthin 
Starry", incidentally, but hey---they're making the big bucks....) I'll
take anyone's 2 cents... Thanks, Lynda Leopold

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