RE: [Histonet] Steiner and Steiner method/H. pylori

From:"connie grubaugh"

I tried to use Zinc Formalin instead of uranyl nitrate and the bugs would 
not stain.  Tried it a couple of times and the Doctors were not happy at all 
with the results.  \\\\
We only use the Steiner when the Doctors think they see bugs but due to a 
lot of inflammation they need to be more specific.
We use the Diff Quick stain  for the daily routine.
Connie Grubaugh
Lab. Med Con
Las Vegas Nv


>From: Tony Henwood 
>To: histonet@lists.utsouthwestern.edu
>CC: "'Derek and/or Lynda Leopold'" 
>Subject: RE: [Histonet] Steiner and Steiner method/H. pylori
>Date: Wed, 10 Dec 2003 09:13:13 +1100
>
>For HP, the Warthin Starry modifications seem to be the best (?gold
>standard).
>Unfortunately many of us find the stain complex and long. Not a good 
>routine
>stain for most labs.
>You can replace the uranyl nitrate with Zinc Formalin, Lead nitrate and
>ferric alum
>
>Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
>Laboratory Manager
>The Children's Hospital at  Westmead,
>Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>Tel: 612 9845 3306
>Fax: 612 9845 3318
>
>http://www.histosearch.com/homepages/TonyHenwood/default.html
>http://us.geocities.com/tonyhenwoodau/index.html
>
>
>-----Original Message-----
>From: Derek and/or Lynda Leopold [mailto:leopold@mnsi.net]
>Sent: Wednesday, 10 December 2003 8:30 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Steiner and Steiner method/H. pylori
>
>
>Hi list,
>Would anyone care to comment on the Steiner and Steiner (microwave)
>method for spirochetes?  I susupect my lab's pathologists are interested
>in it for viewing H. pylori, yet in all the "HP" talk on this list I
>have yet to hear it mentioned.  How does it compare from a tech safety
>standpoint?  As a brand-new tech, I still do crazy things like read the
>safety warnings on ingredients, and that whole uranyl nitrate
>radioactive thing is sort of bothering me.  We use no protective
>equipment when doing this stain (which the doctors still call "Warthin
>Starry", incidentally, but hey---they're making the big bucks....)
>I'll take anyone's 2 cents...
>Thanks,
>Lynda Leopold
>
>
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