RE: [Histonet] PBS

From:"Monson, Frederick "

Afternoon Sharon,
	I routinely make up 10X PBS, autoclave it and store it at room
temp to prevent recrystallization.  I make up what I estimate that I
will need for a month and keep it "for my use ONLY!!!!!"  But then, I
always check the osmolarity and pH of the final PBS prep, AND I
generally filter it just before use.  I am finicky, and this quirk may
possibly explain why I am considered difficult but reproducible.
[Sodium azide is also a good preservative, as long as you are not trying
to preserve the life of the system immersed in the PBS.]


Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone/FAX:  610-738-0437
CASI Page and Scheduling

-----Original Message-----
From: Sharon Cooperman [] 
Sent: Tuesday, December 09, 2003 12:29 PM
To: Histonet
Subject: [Histonet] PBS

Does anyone know why you're supposed to make up 0.2M PBS for 
perfusion or fixation of slides (to make up neutral buffered formalin 
or paraformaldehyde) fresh?  I understand why it's important to make 
up the formalin or paraformaldehyde fresh, but what could happen to 
the PBS (unless something grows in it).  Is it really important to 
make it up fresh?

Sharon Cooperman        	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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